Tryptone Soya Broth is especially suitable for the tube dilution method of antibiotic susceptibility testing. Five to 10ml of blood may be added to 50ml of medium. Anticoagulants such as `liquid’† (sodium polyanethyl sulphonate) or sodium citrate may be added to the broth prior to sterilisation. The superior growth-promoting properties of Tryptone Soya Broth make it especially useful for the isolation of organisms from blood or other body fluids. For this purpose, the broth (with added agar) should be used soon after sterilisation, or, heated and cooled just before inoculation. The addition of a small amount of agar renders the broth suitable for the cultivation of obligatory anaerobes, such as Clostridium spp. Cultures should be examined at frequent intervals, as maximum growth is reached earlier than with less nutritious media and the phase of decline consequently begins sooner. Tryptone Soya Broth may be used for the cultivation of aerobes and facultative anaerobes, including some fungi. Tryptone Soya Broth conforms to formulations detailed in various international pharmacopoeias 1,4,5,6. Due to the inclusion of both Tryptone and Soya Peptone, the medium will support a luxuriant growth of many fastidious organisms without the addition of serum, etc. Sterilise by autoclaving at 121☌ for 15 minutes.Ī highly nutritious versatile medium which is recommended for general laboratory use. 0 D(+)-Glucose monohydrate 2.5 Sodium chloride 5.0 di-Pot assium hydrogen phosphate 2.TRYPTONE SOYA BROTH (Casein soya bean digest medium) EP/USP/JP/BPĪ highly nutritious general purpose medium for the growth of bacteria and fungiĪdd 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Typical composition (g/litre): Peptone non animal origin 20. Growth / 5 days at 20-25☌ - Candida albicans ATCC 2091 (WDCM 00055) Growth / 3 days at 20-25☌ - Staphylococcus aureus ATCC 6538 (WDCM 00032) Reference medium: Tryptic Soy Agar (Escherichia coli, Staphylococcus aureus, Streptococcus pneumoniae, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus epidermidis), SABOURAUD-2%-Glucose Agar (Candida albicans, Aspergillus brasiliensis) Salmonella typhimurium ATCC 14028 (WDCM 00031) Pseudomonas aeruginosa ATCC 9027 (WDCM 00026) Candida albicans ATCC 10231 (WDCM 00054) Candida albicans ATCC 2091 (WDCM 00055) Bacillus subtilis ATCC 6633 (WDCM 00003) Staphylococcus epidermidis ATCC 12228 (WDCM 00036) Staphylococcus aureus ATCC 25923 (WDCM 00034) Staphylococcus aureus ATCC 6538 (WDCM 00032) Inoculum on reference medium - Escherichia coli ATCC 8739 (WDCM 00012) Growth promotion test in accordance with the harmonised method of EP and USP Stability test (7 days, room temperature) If halogenated, they should be collected in container B. P362 + P364: Take off contaminated clothing and wash it before reuse.ģ Relatively unreactive organic reagents should be collected in container A. P333 + P313: If skin irritation or rash occurs: Get medical advice/ attention. P302 + P352: IF ON SKIN: Wash with plenty of water. P272: Contaminated work clothing should not be allowed out of the workplace. H317: May cause an allergic skin reaction.
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